譜析(上海)生物科技有限公司優(yōu)勢(shì)供應(yīng):E.coli Topoisomerase IV Decatenation Assay Kits
2019/10/11 15:52:17
E.coli Topoisomerase IV Decatenation Assay Kits
除用于脫碳反應(yīng)的分析和稀釋緩沖液外,這些物質(zhì)還含有酶和連接的kDNA底物。1 U的topo IV將在1X緩沖液中孵育,總反應(yīng)量為30 l, 37 C,孵育30分鐘,脫羧200ng kDNA。These contain the enzyme and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 μl at 37°C for 30 minutes.
Cat No. | Product | Product description |
D4001 | E. coli Topo IV Decatenation Kit 1 |
100 U of E. coli Topo IV and 20 μg kDNA |
D4002 | E. coli Topo IV Decatenation Kit 2 |
500 U of E. coli Topo IV and 100 μg kDNA |
D4003 | E. coli Topo IV Decatenation Kit 3 |
1000 U of E. coli Topo IV and 200 μg kDNA |
D4004 | E. coli Topo IV Decatenation Kit 4 |
2000 U of E. coli Topo IV and 400 μg kDNA |
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